Beta-nerve growth factor (Protein name
), NGF_MOUSE from NCBI database.
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General Annotation
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Antigen Annotation
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3D
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Predicted Eptitope
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Vaild Sequence
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Related Databases
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Citations (1)
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Gene name:
Ngf(Ngfb);
Protein name:
Beta-nerve growth factor(Beta-NGF);
Alternative:
Organism:
Mouse (Mus musculus).
General Annotation
Sub Unit:
Homodimer.
Function:
Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.
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Precision
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.3%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.5%
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.0%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.3%
Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤3.8%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤7.3%
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Recovery
Recovery was determined by spiking various levels of NGF into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
97
91-100
Plasma
98
95-106
Recovery was determined by spiking various levels of NGF into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
100
94-105
Plasma
101
98-107
Recovery was determined by spiking various levels of NGF into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
99
92-103
Plasma
98
97-105
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Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
89-97%
92-102%
100-104%
91-98%
EDTA plasma(n=5)
90-98%
95-100%
90-96%
99-104%
heparin plasma(n=5)
95-102%
93-104%
97-102%
85-96%
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
100-106%
94-103%
90-102%
97-105%
EDTA plasma(n=5)
87-94%
91-99%
102-107%
103-110%
heparin plasma(n=5)
102-106%
91-96%
97-104%
95-106%
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NGF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
90-100%
97-104%
93-105%
102-107%
EDTA plasma(n=5)
97-101%
102-105%
89-94%
94-103%
heparin plasma(n=5)
102-106%
90-97%
97-103%
92-105%
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Cited for: X-RAY CRYSTALLOGRAPHY (3.15 ANGSTROMS) OF 7S COMPLEX